Primer
Part:BBa_K864445:Experience
Designed by: Arvid Hedén Gynnå, Sabri Jamal, Lisa Branzell, Hampus Elofsson Group: iGEM12_Uppsala_University (2012-09-29)
The primers that were used in order to perform the site directed mutagenesis to replace the antisense region with a random nucleotide sequence showed to contain a problematic secondary structure. Be sure to check https://parts.igem.org/Part:BBa_K864440:Experience to see the PCR program and PCR protocol that was used to bypass the problems with the secondary structure.
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